FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_rootEV_minusN_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_rootEV_minusN_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634137
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC211710.45684881564787577No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA95750.20661883755270938No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA55450.11965550435820088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG45850.067.861576
CTATAGG68750.066.537186
TTATACC56800.066.3634346
CTATACC45050.066.34756
CCTATAC38200.066.059215
CGTATAG63750.066.046065
TTATAGG121100.066.039466
TTATAGC68000.065.932826
CGTATAA62500.065.743015
CTTATAG63000.065.5545655
GGTATAG101350.065.198825
CTATAAC45600.064.933236
GTTATAG104450.064.872165
GTATAGG123700.064.792886
CCTATAA49200.064.663655
TATACCG97100.064.628277
CTATAAG46900.064.625896
GCTATAG62400.064.614415
TATACGG72750.064.4663857
CTTATAC58700.064.275015
Produced by FastQC (version 0.11.5)