FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367959
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC86950.2581682259196148No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA42890.12734715594815732No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA42840.12719869808391374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG44050.068.010795
CTATAGG53750.067.392556
CGTATAG55450.067.156815
TTATAGG97450.067.01626
GGTATAA62500.066.917225
CTATACG34950.066.892796
CGTATAA47300.066.667425
GTATAGG96300.066.5808266
CTATACC32350.066.426926
GCTATAG53650.066.409175
TTATAGC46450.066.380456
CTATAAC33050.066.290746
CATATAG58700.066.1216055
GTATAGC50850.066.005126
GGTATAG82300.065.829585
GTATAAC40250.065.823356
CTATAAG39150.065.795486
GTATAGA62200.065.608216
TATAGGG123100.065.5334247
GGTATAC48850.065.483465
Produced by FastQC (version 0.11.5)