FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4270695
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC135270.31674001538391294No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA72680.17018307324686027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG45650.068.080526
TTATAGC58700.067.910416
CTATAGG71150.067.8820346
GCTATAG66550.067.841015
CTTATAG58450.067.6619645
GGTATAA82250.067.656875
CGTATAG67850.067.36655
TTATAGG134300.067.339086
GTATAAG94700.067.299326
TTATAAG84300.067.2986156
CTATACC38900.067.298086
GTATAGG144750.067.240846
GGTATAG120500.067.208935
CTATAAC42550.067.20066
CGTATAA58600.067.190155
GGTATAC66000.067.133685
TTTATAG105850.066.9552845
CGTATAC40550.066.803745
CATATAG80800.066.661945
GTATAGA90650.066.5996556
Produced by FastQC (version 0.11.5)