FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3439460
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC101990.29652910631320034No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA55940.16264181005157785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG57950.067.279366
CTATAAC33250.067.2603766
CCTATAG31300.067.20175
CTTATAG47200.067.105285
GCTATAG55900.066.991875
CGTATAA43850.066.8045355
TTATAGC50250.066.793266
GTATAGG115100.066.713146
GGTATAA65000.066.658785
CGTATAG54500.066.593585
GGTATAG94150.066.428485
CTATACG34900.066.386956
TTATAGG105750.066.35666
GGTATAC51200.066.2374955
TTATAAG64900.066.222236
GCTATAC36750.066.1877445
GTATAGA69650.066.178176
GTATAAG74700.066.155236
CTATAGC35400.066.0424656
GATATAG70800.065.79535
Produced by FastQC (version 0.11.5)