FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537015
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC112490.31803653645800206No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA62630.17707021315996682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG39000.068.4719246
CGTATAA44550.068.190625
CTTATAG46500.067.7395255
GGTATAA67900.067.729555
CTATAGG61200.067.6528246
TTATAGC48100.067.378076
CGTATAG54700.067.246245
GTTATAA57100.067.1785
CTATAAC33750.066.990216
TTTATAG85650.066.932825
GCTATAC37300.066.901115
CGTATAC36000.066.789295
TTATAGG106550.066.745636
GTATAGC53350.066.71746
GATATAG72350.066.708065
GGTATAG97550.066.6967545
CTATACC33800.066.684016
GTATAAG75650.066.620246
GTATAGG117100.066.530516
CCTATAC25700.066.456785
Produced by FastQC (version 0.11.5)