FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776992
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC118670.31419182248731264No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA43360.11480034906083995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG52650.067.405455
CTATACG36450.067.405446
CTATAGG58450.067.123796
TTATAGC52950.067.0896456
CGTATAG58350.066.6990055
GGTATAA68600.066.579695
TTATAGG110550.066.2305456
GCTATAG53900.066.16695
GCTATAC40650.066.037445
CGTATAC40200.065.9060445
CGTATAA48800.065.838255
CTATAAC38500.065.816276
CCTATAC30950.065.5877465
TATAGGG136050.065.547527
GTATAGG112600.065.5220646
CTATAAG44500.065.4362956
TTATAAG66750.065.3838656
GGTATAC52250.065.3091055
CTATACC39100.065.1643456
TTTATAG87100.064.975155
Produced by FastQC (version 0.11.5)