FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964232
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC168070.33856193667016365No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA106290.2141116692370542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG85800.068.284316
CGTATAA63650.068.073015
CTTATAG67050.067.9618155
CTATACC46000.067.867176
TTATAGC65550.067.808446
CTATACG53150.067.692826
CGTATAG77300.067.55265
CCTATAC36400.067.209175
GGTATAA97050.067.1846545
CGTATAC51000.067.046655
CTATAAC51750.066.954166
TTATAAG91950.066.9144446
GCTATAG81850.066.8760765
GCTATAC52400.066.858335
GGTATAC73250.066.652945
GTATAAG103150.066.5366746
GTATAAC58500.066.527576
TTATAGG153950.066.519266
GTATAGG164350.066.462526
GTATAGC68850.066.43926
Produced by FastQC (version 0.11.5)