FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613415
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC105110.2908882594443207No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA72260.19997703003945022No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA41230.11410258716477348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG59350.067.9344566
CGTATAG55150.067.840745
CTTATAG47100.067.6205755
CTATACG39500.067.517486
CGTATAA46000.067.4876255
CTATAAC37000.067.3498466
GTATAGG120600.067.0963446
GCTATAG59700.067.067185
TTATAGC49650.066.89686
GGTATAC55150.066.825345
GCTATAC41900.066.824285
CGTATAC37850.066.669585
TCTATAG56050.066.626525
TTATAAG66350.066.464236
GGTATAG96650.066.4134145
GGTATAA71900.066.347625
CTATACC35400.066.3403246
CCTATAC27950.066.116595
GTATAAC39800.066.041246
CTATAAG43050.065.933496
Produced by FastQC (version 0.11.5)