FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-p1107-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329168
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC94850.28490601856079356No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA57550.17286601337030755No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA33960.10200746853267845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG34550.067.9721456
CGTATAA46200.067.573965
CGTATAG54950.067.450435
CTATAGG55650.067.293816
GGTATAG85450.066.926255
GTATAGG105800.066.888586
CTTATAG43350.066.8494345
GGTATAA64000.066.826355
CTATAAC34050.066.708956
TTATAGC49100.066.291526
TTATAGG96650.066.195866
GCTATAG55600.066.158315
CGTATAC33550.066.138335
GTATAGA65000.066.0674746
GGTATAC50300.065.8928765
GTATAAG70050.065.851216
GTATACC41750.065.806636
TATAGGG131300.065.786527
CTATACC31350.065.755846
TCTATAG45900.065.57565
Produced by FastQC (version 0.11.5)