FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595723
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC136740.380285133198525No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA44090.12261789909845669No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA37420.10406808310873779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG38450.067.995036
CTATAGG58300.067.83646
CTATACC37800.067.590256
GGTATAA61100.067.1907965
TTATAGG97300.067.119976
CGTATAG56050.067.062795
CTTATAG52250.067.05035
TTATAGC51850.066.960066
GTATAGG99300.066.9312066
GGTATAC50050.066.920755
CGTATAA46350.066.524125
CTATAAC37750.066.38186
CCTATAC30750.066.355425
GCTATAC39650.066.290255
GGTATAG81150.066.2453845
GTATAAG67850.066.232046
CCTATAG35750.066.179525
GCTATAG55650.066.035445
GTATAGC53000.065.9694066
CGTATAC38100.065.955715
Produced by FastQC (version 0.11.5)