FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4569134
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC157170.3439820324814286No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA51810.11339129034079543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG73600.067.619476
CTATACG47400.067.265146
CGTATAG70700.067.125925
GGTATAA76250.066.784115
CTATACC52200.066.644746
GCTATAC49750.066.6203465
CGTATAA60550.066.529035
TTATACC61600.066.304066
CTTATAG65250.066.296095
CATATAG86300.066.144415
TTATAGG127050.066.057866
TTATAGC71750.066.0460366
GTATAAG88000.066.019986
GTATAGG124200.065.9392856
CGTATAC49200.065.871255
GGTATAC66550.065.842485
CCTATAC38300.065.793615
GTATAGC67700.065.757986
CTATAAC44500.065.6714256
GGTATAG103950.065.653845
Produced by FastQC (version 0.11.5)