FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5172632
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC192610.3723636245532255No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA57420.11100731697132138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG56150.068.1284266
CTATAGG84050.067.5832066
CGTATAA69150.067.518325
CGTATAG80700.067.39625
CCTATAC40550.066.9774865
CTATACC52750.066.879976
GTATAGG153600.066.671636
GGTATAC75700.066.6695255
GGTATAA90300.066.665125
CGTATAC52250.066.5152055
CTATAAC54100.066.440236
TTTATAG119550.066.426735
GCTATAC57800.066.425795
CTTATAG72900.066.397645
TTATAAG93950.066.3103266
GTATAGC79600.066.304996
CTATAAG67000.066.2895056
TTATAGG154150.066.252116
TTATAGC75650.066.204676
GGTATAG122200.066.103215
Produced by FastQC (version 0.11.5)