FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637287
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC149910.32327091249689743No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA56680.12222663811836533No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA51190.11038781942976572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG76800.068.037016
CGTATAA59850.067.891395
CGTATAG71750.067.411315
CTATACC45850.067.24866
CCTATAC40200.067.210625
CTATACG51400.067.2048656
CTATAAC48900.067.062136
GTATAGG127350.066.781166
TTATAGC69150.066.757386
GGTATAG105400.066.609625
GGTATAA74800.066.580945
GCTATAG72150.066.5045
GCTATAC52750.066.413795
TATAACG71700.066.384467
TTATAGG131200.066.342016
CTTATAG62750.066.148135
CGTATAC44750.066.0861365
TTATAAG84200.066.006256
GGTATAC64650.065.936435
AGTATAG106700.065.830875
Produced by FastQC (version 0.11.5)