FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5223158
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC168950.3234633147226257No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA66930.12814086803424288No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA65520.12544135176458382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG83100.067.427796
CTATACG53500.067.249326
CGTATAA68150.067.120985
CGTATAG83650.066.984445
TTATAGC76950.066.722156
GTATAGG137750.066.6431056
CTATACC52850.066.487086
GCTATAC61050.066.32795
GGTATAC70550.066.3258745
GGTATAA86400.066.027135
CGTATAC54650.065.834195
GTATAGC83100.065.827386
GCTATAG81500.065.7025765
CTTATAG74950.065.607465
CTATAGC58250.065.1902166
AGTATAG117950.065.189965
TATAACG76900.065.172637
TTATAGG144800.065.162836
TATAGGG174050.065.1507647
GGTATAG112950.065.1320955
Produced by FastQC (version 0.11.5)