FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6527-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023510
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC200570.39926266694004786No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA68440.13623940233024318No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA56510.11249106700295212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG52850.067.8125156
CGTATAG79600.067.359775
CGTATAA64500.067.339055
CTATAGG80950.067.101126
GCTATAC56950.066.617835
CGTATAC55300.066.3903965
GGTATAA81950.066.19695
TTATAGC74500.066.145656
CCTATAC44550.065.9912645
TTATAGG132200.065.920856
GTATAGG139000.065.8936846
CTTATAG71950.065.76595
GGTATAC70750.065.694125
CTATACC53100.065.515916
TTATAAG88700.065.381336
GCTATAG80750.065.316915
CTTATAC61600.065.282115
CTATAAC54100.065.275286
GGTATAG109600.065.207885
GTTATAC66450.065.204955
Produced by FastQC (version 0.11.5)