FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4511815
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC159310.35309515128612323No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA54360.1204836634480802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG72600.067.587916
CTATACC45600.067.0817266
CTTATAG62750.066.986455
CGTATAA58950.066.911045
TTATAGC66500.066.8931356
CTATAAC51050.066.776076
CTATACG48350.066.7409066
CGTATAG71200.066.606555
CCTATAC38150.066.2369465
GCTATAG71700.065.9468155
TTATAGG116650.065.8578346
GCTATAC49000.065.855575
CTATAAG55800.065.795926
TTATAAG80900.065.542316
GTTATAC61150.065.476765
TTATACG70850.065.453626
TTTATAG100900.065.246215
CTATAGC52450.065.060416
CGTATAC46050.064.906165
GTATACC58450.064.848756
Produced by FastQC (version 0.11.5)