FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798775
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC205080.42735906559486536No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA67910.14151528254606643No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA59840.12469849076066288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG50050.068.8083346
CTATAGG80850.068.2655646
TTATAGC69750.068.090366
CGTATAA64500.067.772385
CGTATAG81800.067.515535
CCTATAC40100.067.3787465
ATTATAG105050.067.33185
CTATAAC51500.067.1428456
CTTATAG68050.067.116985
CTATAGC60350.067.097466
CTATACC51400.067.06926
TTATACC65350.067.051546
GCTATAC50900.067.0404365
TTATAGG137100.067.035876
TTATACG74050.067.01956
TTTATAG107150.066.992095
CGTATAC54050.066.953745
GTATAAG90400.066.899876
GGTATAA78900.066.892035
GCTATAG78450.066.8296055
Produced by FastQC (version 0.11.5)