FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308845
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC131620.3977823077236921No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA45680.13805421529264744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG38600.068.093396
CTATAGG57350.067.800516
GGTATAA54700.067.694075
CGTATAA42350.067.518215
CTATAAC34700.067.475946
CGTATAG53250.067.434175
TTATAGC49300.067.299796
GCTATAC36750.066.949955
TTATACG51650.066.880446
CTATACC33550.066.867916
TTATACC48250.066.805866
GCTATAG52500.066.797585
CTTATAG47850.066.6329045
CGTATAC36250.066.618285
GTATAAG66050.066.606216
CCTATAC25800.066.606115
GGTATAC49100.066.5760045
TTTATAG81000.066.497585
GTATAAC36300.066.43016
CTATAGC39000.066.407856
Produced by FastQC (version 0.11.5)