FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5213046
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC226230.43396893102420353No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA72710.13947699675007663No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA69920.1341250393723746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG61000.068.563746
CTATAGG88850.068.3043066
CTATAAC55100.068.029046
GGTATAA87700.067.922935
CGTATAC56100.067.814595
CGTATAG92550.067.766965
CTTATAG73650.067.7646565
GCTATAC59350.067.757255
CGTATAA69000.067.6648565
TTATAGC77000.067.634556
CTATACC54100.067.6044856
CCTATAC43150.067.564825
CTTATAC65700.067.547665
TTATAAG99550.067.431656
GTATAAG99700.067.3652956
GCTATAG83450.067.313965
CCTATAA56250.067.260435
TTATACC69900.067.19426
CTATAAG68100.067.171486
TTATACG82300.067.148876
Produced by FastQC (version 0.11.5)