FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298027
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC126030.3821375628519718No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA47190.14308554781388994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA42500.068.186615
CGTATAG54350.068.002065
CCTATAC25800.067.6921845
CTATAGG55500.067.41286
GCTATAG50350.067.148355
CTTATAG46550.067.141265
CTATACG33600.067.081746
GGTATAA56300.067.076555
TTATACG50000.067.05846
CTATAAC35650.067.05316
TTATAGG94000.066.870756
TTTATAG82000.066.711825
CGTATAC35500.066.547715
GTATACC41850.066.485876
GTATAAG66200.066.456126
TTATAGC51100.066.4367756
GTATAGA62050.066.3320166
TTATACC42900.066.3270956
CTATACC33350.066.325256
GTTATAC40350.066.268565
Produced by FastQC (version 0.11.5)