FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-6168-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5191368
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC216170.4164027670548495No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA77700.1496715316656419No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA60580.11669371156119157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG55450.068.356646
CGTATAG85650.067.627685
CTATAGG83350.067.604266
CCTATAC43400.067.4171455
TTATAGC76000.067.234646
CTATAAC55750.067.172696
GCTATAC56650.067.1557855
CTATACC54650.067.115826
CTTATAG73750.067.055435
GCTATAG81000.066.92995
GGTATAA89250.066.782135
CTATAGC59100.066.6221856
GGTATAC73800.066.4883355
TTATAAG93250.066.469676
CGTATAC56050.066.43855
TTATACC70800.066.339636
CGTATAA68400.066.313625
TTATAGG150550.066.231646
GTATAGC76150.066.137046
TTTATAG126000.066.136735
Produced by FastQC (version 0.11.5)