FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894022
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC152400.39136912939885804No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA54090.1389052244697128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC41250.067.198236
CGTATAG56050.067.063355
TTATACC54350.067.035956
CCTATAC33050.066.9271245
CTATAGG65800.066.48766
TTATAGC60450.066.46646
CTTATAG56050.066.376485
TTATAGG106300.066.08016
CGTATAA50800.066.071095
CTATACG44000.065.702816
GGTATAA61400.065.6660165
GTATAGG101200.065.6405646
GCTATAC43050.065.445435
GGTATAC50700.065.234965
GTTATAA65600.065.196455
GTATAAG73150.065.165756
CCTATAA45550.065.080615
GCTATAG60450.065.076865
ATTATAG81750.065.074745
TATACCG75800.065.057657
Produced by FastQC (version 0.11.5)