FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549290
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC102490.2887619777476622No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA68740.1936725373243663No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA66520.18741776524318948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG58950.068.276696
CTTATAG49950.067.756265
CGTATAA49850.067.611345
CGTATAG59800.067.364735
CTATACG42050.067.002086
GGTATAC52150.066.9783865
CCTATAG32400.066.973825
GGTATAA66350.066.727985
TTATAAG60650.066.651456
CTTATAC42200.066.515125
GTATAGG105450.066.5134666
GCTATAG59950.066.2037055
GTATAAG74600.066.104436
CCTATAC29200.066.043055
TTATAGG100950.066.011426
CTATACC34350.065.922856
GTATAAC41050.065.9059756
TTATAGC51500.065.852916
GGTATAG85900.065.8425455
TTTATAG77850.065.817425
Produced by FastQC (version 0.11.5)