FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3102399
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC66480.21428578335668624No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA51820.16703202908458906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA40650.066.8127445
GCTATAC32650.066.6753545
GGTATAA59650.066.477955
CTATAGG50300.066.4497766
CTTATAG43350.066.3652655
GTATAGG95600.066.3373956
CGTATAG44400.066.293535
TTATAGG89650.066.289626
GTTATAA50300.065.893135
CCTATAC24050.065.778125
CTATACC30150.065.7033166
GGTATAG80200.065.6780555
TTATAAG58350.065.559786
GGTATAC42600.065.479735
TTATAGC44050.065.469566
CTATACG32800.065.1966866
TATAACG46150.065.068947
TATAGGG118200.065.02397
CTATAAC31850.064.943576
GTATAGA56000.064.811036
Produced by FastQC (version 0.11.5)