FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3991716
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC141840.3553359006502466No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA50380.12621138377579968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA48650.067.336195
CTATAGG68000.067.3215946
CGTATAG60150.067.205055
TTATAGC61700.066.764696
CTATACC43600.066.7067956
GCTATAG62300.066.683475
CCTATAC34700.066.6695565
GTATAAC41600.066.63276
CTATACG45100.066.583456
TTATAGG109950.066.432836
CTTATAC52150.066.3739245
CTTATAG56450.066.339985
GCTATAC43900.066.091495
GGTATAA63650.065.9839555
GTATAGG113150.065.8531656
TTATACC59000.065.8455666
GGTATAC55750.065.7918245
CTATAAC44550.065.7556846
CCTATAA45150.065.7345355
CTATAAG49550.065.689336
Produced by FastQC (version 0.11.5)