FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372406
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC110270.32697723820915986No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA58910.1746824077528032No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA52780.15650547413330423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA46350.068.412895
CTTATAG46300.068.411195
CTATAGG59300.068.2870256
GGTATAC52200.068.255365
GCTATAG56150.068.2533345
CTATACG37400.068.220586
GCTATAC40250.068.085625
GGTATAA61700.067.8997965
TTTATAG69700.067.8896265
GTATAAG70100.067.851736
AGTATAG81250.067.844825
CTTATAC38700.067.647255
CGTATAG58300.067.59735
GTATAGG116200.067.5589146
TTATAAG61400.067.490536
CTATACC33350.067.4799356
CCTATAC26400.067.479735
TTATAGC48350.067.465066
CATATAG63600.067.412195
GATATAC41900.067.409175
Produced by FastQC (version 0.11.5)