FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5780-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812757
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC63880.22710813625208293No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA49110.17459737901283331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG47200.068.07026
CGTATAG43100.068.049215
GTATAGG91050.067.960836
CTATAAG36500.067.600926
GCTATAC29400.067.498185
TTATAGC39900.067.454326
CGTATAA36950.067.3459555
GGTATAG75800.067.135395
GGTATAA55550.067.03695
CTATACG28550.066.8108146
CTTATAG37000.066.68745
TTATAGG87900.066.4544146
AGTATAG69400.066.367095
CGTATAC28450.066.307535
CCTATAC20700.066.278415
TTATAAG56200.066.261566
CTATACC27150.066.259736
GTATAGA53400.066.1967246
ATTATAG63650.065.819125
GTATAGC43800.065.763076
Produced by FastQC (version 0.11.5)