FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888656
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA80270.20642093309359327No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA58340.15002612727893647No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC44130.11348393892388527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG54650.065.8341146
GGTATAA71650.065.3075265
CTATACG38400.065.075136
CGTATAG55300.064.427415
TTATAGG102950.064.217536
GGTATAG99950.064.1841355
TTATAGC59300.064.153886
CTATACC34900.063.979866
GTATAGG107500.063.8435676
CTTATAG50300.063.8042145
CCTATAC30700.063.72675
TATAGGG114500.063.638997
GGTATAC55350.063.4207425
GCTATAC44550.063.319195
CGTATAA50250.063.3105135
GTATAGC62800.063.1977276
CTATAAC34350.063.1703036
TATAACG45300.062.6571547
TTTATAG93350.062.610935
TATAGGC82500.062.530457
Produced by FastQC (version 0.11.5)