FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4916752
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA95070.1933593559325343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG122250.064.8159565
CTATAGG65950.064.690916
GTATAGG127900.064.5796666
CTATACG45500.064.2287456
TATAGGG125050.064.1483157
CGTATAG66300.063.135265
TTATAGC72950.062.9452446
TTATAGG117650.062.8581966
GTATAGC76450.062.7645346
CGTATAA68350.062.4705965
TATAGCG78450.062.4581957
CTATACC41850.062.303896
GGTATAC67650.062.2892385
GGTATAA95800.062.21625
GTTATAG121500.062.1338425
CTATAAC44550.061.9845736
CCTATAC41250.061.8525925
TATAACG49800.061.775167
GCTATAG78200.061.7180025
TATAGGC96000.061.5397267
Produced by FastQC (version 0.11.5)