FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690094
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC104460.28308221958573415No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA73910.2002930006661077No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA53750.1456602460533526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG41900.068.743786
TTATAGC53450.068.687136
CGTATAG58900.068.630055
CTATAGG68200.068.4058766
GCTATAG61250.068.225365
GGTATAA65500.067.8594065
TTATACC44750.067.806866
CTATAAC39300.067.681296
GGTATAC56050.067.4990545
ATTATAG84450.067.4687045
CCTATAG35500.067.433455
CTTATAG49800.067.326155
GTATAAG76750.067.306286
GCTATAC42700.067.2919165
TTTATAG86800.067.25495
CGTATAA50800.067.240945
CGTATAC37850.067.222735
CTATACC33650.067.188526
CTTATAC42350.066.856355
GTATAGC56500.066.837566
Produced by FastQC (version 0.11.5)