FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3506714
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC92130.2627245905996326No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA62430.1780299163262245No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA45450.1296085166911245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG60200.067.846616
CGTATAG57350.067.7397165
CTATACG40150.067.644116
TTATAGC50150.067.345756
CGTATAA47200.067.32835
GGTATAA67200.067.081145
GCTATAG57500.067.076065
GTATAGG114500.067.032746
TTTATAG79500.067.004095
CCTATAC26600.066.971495
GCTATAC39700.066.912165
CTATAAC35900.066.780546
CGTATAC36950.066.493095
TTATAGG110400.066.44716
GGTATAG90650.066.407095
TTATAAG65000.066.390146
GGTATAC51550.066.331485
CTTATAG48100.066.141285
CTATACC31000.066.046226
ATTATAG78450.065.937935
Produced by FastQC (version 0.11.5)