FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3851163
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC97550.2533001070066367No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA72730.18885204287639865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG67450.067.818956
TTATAGC57250.067.797476
CGTATAG63450.067.791885
GGTATAC57150.067.732375
CTATACG41000.067.693466
GCTATAG65750.067.3899845
GGTATAA72650.067.300485
GTATAGG128500.066.838836
CGTATAC42050.066.752235
CTTATAG49400.066.668415
CCTATAC31150.066.627585
TTATAAG69850.066.591076
CATATAG72400.066.324355
GTATAGC60300.066.1675346
GGTATAG103300.066.101965
GTATAGA76550.066.0663156
CTATACC36350.066.050656
TTTATAG90300.065.967385
GCTATAC43000.065.928625
AGTATAG95050.065.837425
Produced by FastQC (version 0.11.5)