FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5699-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761823
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA71860.191024404922826No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC71760.1907585763604508No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA55140.14657786929369085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG39800.067.888266
CGTATAG61650.067.7847065
CTATAGG66750.067.69176
CTATAAC34550.067.668866
CGTATAA50650.067.511165
GTATAGG122050.067.4752666
TTATAGC52950.067.420916
GGTATAA71550.067.406235
GGTATAC56050.067.2512745
CCTATAC28850.066.844595
CTTATAG50550.066.744645
GGTATAG100250.066.717045
GCTATAG59350.066.696395
CGTATAC39250.066.342795
GTATAGC58950.066.139646
TATAGCG71300.066.022697
TTATAGG111500.065.792576
TTTATAG88150.065.750415
GTATAAG78850.065.7375956
GCTATAC42150.065.680945
Produced by FastQC (version 0.11.5)