FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4567337
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC116680.2554661501877352No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA62380.13657849201843436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG78450.067.9902346
CTATACG48250.067.604116
GGTATAA89150.067.5638055
CGTATAA54650.067.500185
CTATAAC41800.067.402216
GTATAGG144750.067.289796
GGTATAG120650.067.2420045
TTATAAG88000.067.213826
GCTATAC49350.067.1610265
TTATAGC66500.067.103176
CGTATAG70950.067.038085
CTATACC43850.066.964856
CTTATAC53400.066.917395
TTATACG67800.066.900576
CTTATAG60500.066.816115
GGTATAC64000.066.7166755
TTATAGG134850.066.519996
GTATAAG102300.066.3713466
GATATAC53850.066.29325
TTTATAG106700.066.225685
Produced by FastQC (version 0.11.5)