FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582166
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC102760.2242607535388286No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA58330.12729787615725838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG73900.067.630375
GTATAGG154500.067.212046
CGTATAA57250.067.125095
CTATACG47200.067.10656
TTATAGC62750.067.098056
CTATAGG77100.067.047746
GGTATAA90300.066.820165
CTATACC40600.066.7225956
CTATAAC45300.066.5216456
GGTATAG126300.066.5067755
GCTATAG74550.066.477345
GCTATAC45250.066.363115
GTATAAG104650.066.352986
CTTATAG59150.066.211495
GGTATAC66500.066.051115
CCTATAC31600.066.011145
GTTATAC60350.065.996825
TTATAGG145950.065.84986
TTTATAG110650.065.823155
TATAGGG206250.065.705157
Produced by FastQC (version 0.11.5)