FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094449
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC125920.3075383281120366No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA86290.21074874787791958No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA58380.14258328776350615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG43750.069.0381556
CTATAGG68650.068.264756
CGTATAG68550.067.955885
GTATAGG133300.067.6876146
GGTATAG108250.067.508595
TTATAGC58400.067.481086
CTATACC37600.067.205656
GCTATAG63400.067.182755
CTATAAC43000.067.149376
GCTATAC46850.067.0846565
CCTATAC29600.067.042135
GTATAGC59650.066.947086
TATAACG71600.066.869727
GGTATAA80500.066.867785
CGTATAA53600.066.8638845
GGTATAC64350.066.734815
CATATAG75200.066.554085
TTATAGG125300.066.4786766
GTATAAG85250.066.467436
TTTATAG97950.066.460715
Produced by FastQC (version 0.11.5)