FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341261
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC71540.16479082920837976No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA57670.13284158681083677No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA49310.11358450920135879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG63450.066.358265
CTATAGG67000.066.2377246
CTATAAC42750.065.7416156
CCTATAC34850.065.680425
GTATAGG122400.065.566756
TTATAGC65800.065.477656
CTATACC38600.065.283916
CGTATAA59150.065.206045
CTATACG45550.065.081276
GGTATAG107250.064.940675
GGTATAA76750.064.891455
TATAGGG146900.064.495217
TATAACG55400.064.1866767
GTATAGC69450.064.102636
GGTATAC61600.063.86265
TTATAAG74650.063.8569646
GCTATAG67700.063.5366335
TATAGTC91550.063.4997837
TTATAGG122050.063.3172846
TCTATAG66750.063.2873575
Produced by FastQC (version 0.11.5)