FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5150639
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC111920.21729342708739635No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA71800.1394001792787264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG84300.067.092026
CGTATAG80550.067.000175
TTATAGC71350.066.956966
CTATACG51750.066.954836
GTATAGG165200.066.862726
GGTATAA93550.066.668555
CGTATAA66800.066.5926365
GGTATAG141150.066.502045
CTATAAC50600.066.2631456
TTATAAG99500.066.234516
GCTATAC51350.066.1132355
CCTATAC39600.066.021065
CTTATAG63300.066.017295
GCTATAG83900.065.993565
TTATAGG157650.065.8689356
TATAACG75250.065.440217
TTTATAG125150.065.383895
TATAGGG212250.065.3481757
GGTATAC76000.065.300985
GTATAGC80350.065.2503746
Produced by FastQC (version 0.11.5)