FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-5678-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408056
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC99920.29318767062513057TruSeq Adapter, Index 6 (100% over 50bp)
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC99020.2905468689481628No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA70990.208300567831045No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA57430.1685124892313976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG53550.068.952966
GCTATAC41250.068.3866045
TTATAGC47350.068.002956
CGTATAA47350.067.7812045
CTATACG37800.067.776516
GGTATAC51300.067.679055
CTATACC32100.067.599986
GCTATAG51600.067.556885
CGTATAC38100.067.426565
CCTATAC27350.067.311365
CTTATAG45450.067.3034745
CGTATAG52950.067.288645
GTATAGG102700.067.136046
TTTATAG76600.067.120165
CTATAAC33850.066.8968356
TTATAAG63850.066.819436
GGTATAG86750.066.730745
GTATAGC48350.066.596486
GGTATAA65350.066.571075
GTATAAC38450.066.539726
Produced by FastQC (version 0.11.5)