FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874035
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC118490.3058568133741693No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA48530.12526990592495937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG62500.067.591286
CTATACG39750.067.533876
CGTATAA50100.067.204875
GGTATAA65100.066.8810045
GGTATAC53900.066.817475
CGTATAC41300.066.7789465
TTATAGG115850.066.61566
GCTATAC42150.066.594785
CCTATAC29650.066.576015
CTATACC39100.066.508246
GTATAGG116900.066.49636
CTTATAG50450.066.4611365
GCTATAG64100.066.450155
TTATAGC53950.066.431176
GTATAAG77300.066.3770456
CGTATAG59050.066.265175
GTATAGC57500.066.164516
TTTATAG88250.066.033295
CATATAG72400.065.938525
AGTATAG93900.065.936465
Produced by FastQC (version 0.11.5)