FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5331883
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC191920.35994788332752237No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA67140.1259217428439446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG82050.067.8654865
CTATACG57750.067.331676
CTATAGG85950.067.107136
TTATAGC75400.067.0739446
CGTATAA69350.067.071175
TTATAGG152850.066.8841866
CTTATAG74900.066.493685
CTATACC55850.066.488966
GTATAGG162250.066.439036
GGTATAC73650.066.291655
GTATAGC78850.066.2697756
GGTATAA97050.066.1395345
CTATAAC54100.066.116676
CCTATAC46300.066.067495
GTATAAG111200.065.96966
TTATAAG97850.065.849176
TTATACG80950.065.8044366
CTATAAG69400.065.66126
GCTATAC56450.065.658265
TATAGGG191400.065.5913857
Produced by FastQC (version 0.11.5)