FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-plusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4741651
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC156440.32992727638537717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT100710.21239437487069376TruSeq Adapter, Index 18 (97% over 40bp)
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA60680.12797230331797932No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA53590.1130197055835615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG79300.067.967866
TTATAGC68050.067.6836556
CGTATAG73400.067.137115
CGTATAA61600.067.1004265
GGTATAA80150.066.941385
CTATACG49400.066.8807456
CTATAAC47000.066.796036
TTATAGG127200.066.7513056
GTATAGG128700.066.4900056
CTTATAG67450.066.417745
GGTATAG104450.066.245185
GCTATAG74500.066.0988545
GGTATAC66100.065.7621845
CCTATAC37700.065.6347965
CCTATAG46300.065.613745
TTTATAG109850.065.601295
GTATAAG85400.065.571966
TATAGGG154700.065.5638057
CTATACC47900.065.540996
CTATAAG58650.065.4628146
Produced by FastQC (version 0.11.5)