FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189032
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC90960.28522761765952803No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA42670.13380235758060754No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA33490.1050161930015127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA41750.068.06975
CTATACG34700.067.476266
CTATAGG54050.067.3429266
CGTATAG47500.067.271565
GTATAGG93100.066.764816
TTATAGG92400.066.5130546
TTATAAG58400.066.4619456
CCTATAC27550.066.31375
TTATAGC45000.066.18686
TTTATAG75250.066.0909355
CTTATAG45200.065.971375
CGTATAC31250.065.965925
GGTATAA58350.065.919085
GCTATAG53800.065.704255
GTATAAG64700.065.5620656
TATAGGG118100.065.522917
GGTATAC45300.065.516695
CTATACC31150.065.503556
GGTATAG79100.065.484665
CTATAAG39850.065.430816
Produced by FastQC (version 0.11.5)