FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4987743
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC156390.3135486331192285No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA67820.1359733250089269No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA59800.11989390792588953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG83800.067.701556
CTATACG53100.067.230346
CTATACC50550.067.159936
CGTATAG75800.067.043565
CGTATAA70200.066.957395
TTATAGC71150.066.653666
GCTATAC57400.066.52315
GTATAGG140850.066.470146
CCTATAC37400.066.4425665
CTATAAC49050.066.430926
GGTATAG115650.066.276285
GGTATAA88300.066.2331545
GGTATAC72500.065.991825
GCTATAG78750.065.954285
TTATAGG136300.065.94156
TTATAAG89300.065.609046
CTTATAG70850.065.602115
TATAACG76500.065.560837
GTATAGC74000.065.458196
CGTATAC52800.065.424875
Produced by FastQC (version 0.11.5)