FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmTARGET.0619-1759-minusN-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260610
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC128270.3010601768291395No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA93610.21971032316968697No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA51280.12035835244249063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG42550.066.543046
CGTATAA61350.066.517775
CGTATAG59800.066.369045
CTATAGG63350.065.9646766
GTATAGG112400.065.482716
CTATACC39850.065.430716
TTATAGC62750.065.3683556
TTATAGG105000.065.364516
CTTATAG56150.064.824225
GGTATAC60850.064.82125
GTATAGC60200.064.7653056
CCTATAA47700.064.641475
TATAGGG120450.064.622197
TTATACC48050.064.607656
CCTATAC37250.064.54825
TATACGG82500.064.185767
TTTATAG99350.064.0794145
GGTATAA76000.063.9649965
GGTATAG95550.063.9539265
CTTATAC55850.063.856445
Produced by FastQC (version 0.11.5)