FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose5_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose5_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412478
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA74930.21957650715989963No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC37570.11009594787131229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG34600.067.874156
CCTATAC28150.067.3874365
CTATAGG48300.066.302826
CTATACC35400.065.747076
TTATAGG86750.065.076316
GGTATAC41200.064.986375
CTTATAG45000.064.865175
TTATAGC51850.064.665826
TATACGG47700.064.642127
CGTATAG45250.064.584155
GCTATAC38800.064.495935
GTATAGG86250.064.4796756
CGTATAA39600.064.341965
TTATAAG58650.064.09066
TATACGC42650.064.0899667
TATAGTC60800.063.781437
CTATAAC32600.063.4494636
ACTATAC36450.063.2770085
CCTATAG30600.063.250185
TATAGCG53950.063.1866847
Produced by FastQC (version 0.11.5)