FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294692
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA67440.20469288176254413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG32400.067.18916
CTATAGG46450.065.62756
TTATAGG79550.065.202096
CTATACC29900.064.964376
CTTATAG41800.064.638985
GTATAGG84850.064.305446
TATACGG48800.064.260137
CCTATAC25600.064.118945
TATAGTC55650.063.834337
TTATAGC51050.063.8274356
TCTATAG49850.063.819325
GCTATAC36450.063.756435
TTTATAG76750.063.4766965
TATAGCG49250.063.317677
TATAGGG86400.062.9492727
CGTATAG43550.062.9255525
TATAGGC60800.062.917297
TTATAAG57450.062.8699536
TATACGC35700.062.8410227
CGTATAA40250.062.78055
Produced by FastQC (version 0.11.5)