FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708237
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA60150.16220646091390598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG36850.066.484786
CTATAGG47700.066.256896
CCTATAC30200.065.015615
GTATAGG89400.064.948716
CTTATAG47600.064.925525
TTATAGG88700.064.829946
CGTATAG47500.064.620115
CTATACC35400.064.5611956
GCTATAC41700.064.459495
TATAGCG54050.063.9768647
TTATAGC57400.063.840536
TATACGG55650.063.458197
GGTATAC49650.063.3021975
GGTATAG86450.063.156975
TATAGCC39800.063.0518467
CTATAAC38750.062.5926326
TATAGGG90900.062.5678447
TATAGTC61100.062.3804327
TATACGC39000.062.281147
TATAGGC66250.062.233057
Produced by FastQC (version 0.11.5)