FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose2.5_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose2.5_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537529
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA52890.14951114181678793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG87700.065.248916
TTATAGG87150.065.098456
CTATACG31750.065.037426
TTATAGC54050.064.9471746
CTATAGG48400.064.936086
CCTATAC27100.064.315425
CTTATAG46150.064.158435
GGTATAG82050.063.7701845
TATAGGG93350.063.699227
GGTATAC45900.063.5928735
TATAGCG55700.063.4002577
CTATACC32250.063.2694246
TATACGG52450.062.9914447
CGTATAG45300.062.735435
TTTATAG82100.062.5803455
TATAGCC35100.062.4197927
AGTATAG63250.062.3617745
CGTATAA41200.062.267565
CTATAAC34350.062.2543266
TTATAAG58250.061.9466486
Produced by FastQC (version 0.11.5)