FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose2.5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose2.5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936383
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA109650.22212620049943452No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC54510.11042498120587482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG49200.066.511946
CTATACC48250.066.080636
CCTATAC40200.065.905695
CTATAGG72350.065.885776
GTATAGG126400.065.068966
CTTATAG65900.064.7929155
GGTATAC63350.064.417685
TTATAGG126800.064.311676
TATAGCG72850.064.232517
TATACGG75050.064.028447
TTATAGC78200.063.8213356
CTATAAC47000.063.519096
CGTATAG66100.063.379065
AGTATAG85550.063.3701135
TTATAAG85050.063.04316
GCTATAC53350.062.978155
CGTATAA57150.062.954965
GGTATAG115400.062.8097955
TATAGGG129900.062.7230157
TTATACG74350.062.418836
Produced by FastQC (version 0.11.5)